CAP-DNA COMPLEX SHOWN AS ORIENTED HETERODIMER

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4. Transcription activation by pre-recruitment.

Recruitment vs. post-recruitment. Ptashne and Gann, Nature 386:569-577, 1997. These two mechanisms are summarized and examples are given.

Recruitment is the mechanism by which MelR activates transcription of the melAB promoter . Grainger et al. J. Bacteriol. 186:6938-6943, 2004. Convincing in vivo evidence for recruitment using chromatin immunoprecipitation (ChIP).

Initial evidence for pre-recruitment as a new mechanism of transcription activation. Griffith, Shah, et al., Biochem. Biophys. Res. Commun. 291:979-986, 2002. What is the key argument supporting the existence of the pre-recruitment mechanism? Are there alternative explanations and how can the model be tested?

Genetic evidence for pre-recruitment. Griffith and Wolf, J. Mol. Biol. 344:1-10, 2004. What is the genetic evidence? How good is it? What else is needed? Print out the packet that summarizes the background to this work and describes recent work demonstrating that wild type SoxS and DNA binding mutants of it form binary complexes with RNA polymerase in vivo but a positive control mutant of SoxS does not.

Target site on RNA polymerase for interaction with SoxS during binary complex formation and the physiological consequence of this interaction. Shah and Wolf, J. Mol. Biol. 343:513-532, 2004. What is the yeast two-hybrid system and how was it used to identify the protein-protein interactions between SoxS and RNA polymerase? Does data from this system assure that the same interactions occur in E. coli? What is the affinity immobilization assay? What role does induction of SoxS synthesis play in regulation of rRNA synthesis during oxidative stress? Does this make sense physiologically? In other words, does this "redeployment" increase the chance that the cell will survive the stress?