Investigation of light, temperature and cultivated modes on growth and artemisinin synthesis
of Artemisia annua L. shoots

Liu-Chun-Zhao; Wang-Yu-Chun {a}; Kang-Xue-Zhen; Ouyang-Fan; Ye-He-Chun; Li-Guo-Feng
 

The effects of light, temperature and cultivated modes on growth and artemisinin accumulation of
Artemisia annua L. shoots (Fig. 1) were investigated. The time course of shoots growth is shown
in Fig. 2. Figs. 3 and 4 show that the artemisinin yield in shoots were enhanced under light irradiation
length of 20 h/d with light intensity above 3 000 lx. The optimum temperatures for growth and
artemisinin accumulation were 25degreeC and 30degreeC respectively (Fig. 5). The artemisinin
yield was improved in a bi-staged temperature culture (25degreeC for 25 days and then 30degree C
for 5 days) (Fig. 6). It was of beneficial to obtain the maximum artemisinin  yield in shake flask culture
with a shallow layer of liquid and low rotation (Fig.    7).