Welcome to the buffer design website.  You need to install and load Solver in your Microsoft Excel to use this software.  Please click to download the buffer design software.

If you prefer to use our calibration experimental data,  only parameters in steps 2 and 3 of part 3 can be changed in the software.  If you find it difficult to enter reasonable parameters in step 2 of part 3, here are some example sets of parameters you can use to get a linear pH gradient:
 

Example No.	Column length (cm)	Flow rate (mL/min)	Midpoint slope (pH unit/sec)	Transit time (sec)
1	3.5	0.5	-0.004  to -0.003	200   to   250
2*	3.5	1.5	-0.015   to  -0.01	100   to   120
3**	7.5	1	-0.0045  to  -0.0035	150   to   200
4	6	1.2	-0.005  to  -0.004	150   to   200

*Change the maximum value of the objective function F in Step 3 of Part 3 to 0.002.

**If the solver says that it could not find a feasible solution, keep the solver solution as a new starting point and try solving the problem again.
 
 

Questions? Comments? Contact us at xkang@gl.umbc.edu