Beginning Molecular Biology Laboratory Manual

Department of Biological Sciences

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CHAPTER 1: General Laboratory Methods

    Safety Procedures
    Preparation of Solutions
    Disposal of Buffers and Chemicals
    bullet Micropipets
    bullet Using a pH meter
    bullet Autoclave operating procedures
    bullet Operating instructions for spectrophotometer
    Working with DNA
    Sterile Technique

CHAPTER 2: Instructions for Notebook Keeping

CHAPTER 3: Computer User's Guide

CHAPTER 4: Molecular Biology Methods

M.1: Preparation of genomic DNA from bacteria
M.2: PCR amplification of DNA
M.3: Restriction enzyme digestion of DNA
M.4: Phenol/chloroform extraction of DNA
M.5: Ethanol precipitation of DNA
M.6: Agarose gel electrophoresis
M.7: Transformation of E. coli by electroporation
M.8: Wizard PCR preps DNA purification system
M.9: Alternate method for purifying DNA from agarose gels
M.10: Transfection of mammalian cells using Lipofectamine (LTI)
M.11: Southern blotting
M.12: RT-PCR Protocol
M.13: Preparation of sequencing gels
M.14: Isolation of RNA from mammalian cells using RNAZOL (Teltest)

CHAPTER 5: Tissue Culture Methods

Types of cells grown in culture
Work area and equipment
Preservation and storage
Safety considerations
Tissue culture methods
Determining cell counts

This Web page is maintained by Julie B. Wolf, UMBC;
Last updated on 11/23/2005