preliminary effort of applying GFP on high-throughout screening

Monitoring gene expression in yeast:

Note: With the convenient and real time measurement of GFP expression, there is a great potential for applying it in high-throughout screening. The studies in E. coli has shown that for drug screening. Here we look at a prelimany effort on its potential in yeast. The result shows that galactose concentration effect on GFP expression level followed the same trend for both cultures grown in shake flasks and in the 96 well plates. There was a higher standard deviation observed in the plate cultures as compared to the shake flask studies. This was probably due to poor mixing in the plate cultures. The important factor is the tremendous labor savings brought by the plate studies. The plate experiment allowed essentially 96 independent experiments, about 4 times more than with shake flasks, and the plate reader finished each measurement in a few minutes whereas it took more than one hour to do just one fluorescence intensity measurement manually for each of the flasks.

Reference:

Li J, Wang S, VanDusen WJ, Schultz LD,George HA,Herber WK, Chae HJ, Bentley WE, Rao G. 2000 Green Fluorescent Protein in S. cerevisiae: Real Time Studies of the GAL1 Promoter. Biotechnol. Bioeng. (In press)