Bioprocess control and monitoring
back to Quantitative monitoring of gene expression
Monitoring gene expression in yeast:
Note: An example that shows how GFP could be used for
quantitative promoter study. In this study, the effect of initial galactose
(inducer) concentration on GFP expression kinetics was exploited.
It showed that GFP expression was slow at low initial [gal], the expression
rate increased with increasing initial [gal] and reached the maximum expression
above certain initial [gal] (about 1 g/L in this case).
Reference:
- Li J, Wang S, VanDusen WJ, Schultz LD,George HA,Herber WK, Chae HJ, Bentley WE, Rao G. 2000 Green Fluorescent Protein in S. cerevisiae: Real Time Studies of the GAL1 Promoter. Biotechnol. Bioeng. (In press)