ENCH772 course

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Introduction of GFP

General Applications of GFP

• Attributes of GFP
• Green mouse?
• Links to application

Quantitative monitoring of gene expression

• Why quantitative
• GFP sensor

• Bioprocess control and monitoring
• High-throughout screening

GFP is the representative of a new protein folding, which Yang et al. have named a beta-can. On the outside, 11 antiparallel beta strands (green) form a very compact cylinder. Inside this beta-structure there is an alpha-helix (light blue), in the middle of which is the chromophore (yellow). There are also short helical segments (brown) on the end of the can. The cylinder has a
diameter of about 30 Å and a length of about 40 Å

References:
Yang, F., Moss, L. G., Phillips, G. N. Jr., Nature Biotech. (1996) 14, 1246-1251