GFPhistory

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ENCH772 course

Main page

Introduction of GFP

General Applications of GFP

Attributes of GFP
Green mouse?
Links to application

Quantitative monitoring of gene expression

Why quantitative
GFP sensor

Bioprocess control and monitoring
High-throughout screening

Reference & Links

Jellyfish Aequorea victoria: one of the oldest species on the earth

How it fluoresce in nature:
Step1.Calcium binds to photoprotein aequorin; Step2. Aequorin emits blue light
Step3. GFP absorbs blue light; Step4. GFP emits green light

History:
Discovered by Shimomura at 1962, cloned by Prasher at1992.
Now different color mutants available: blue, yellow, cyan
Russian scientists discovered red fluorescent protein from deep sea coral in 1999