Correct answers are in bold

1. You have two pea plants, one of which is homozygous dominant (AA) for a gene controlling pea color, and another which is homozygous recessive for the same gene (aa). You cross the two plants and collect the progeny plants. You allow one of these plants to self-pollinate and collect the progeny plants. Which of the following is true about those plants?
a. They are all identical in genotype
b. They are all identical in phenotype
c. Those with the dominant phenotype outnumber those with the recessive phenotype by 3:1
d. Those with the recessive phenotype outnumber those with the dominant phenotype by 3:1
e. The plants are all sterile


2. You have a plant that is tall with red flowers which you allow it to self-pollinate. You collect the seeds and grow plants from them. You find that the plants which develop are of several types: tall with red flowers, short with red flowers, tall with white flowers, and short with white flowers. These types arise in a ratio of 9:3:3:1. Assuming that a gene called T controls plant height and R controls flower color, what is the genotype of the original plant?
a. Can't be determined from the available information
b. RR TT
c. Rr TT
d. RR Tt
e. Rr Tt

3. Gregor Mendel's theory of genetic inheritance assumes several things about the behavior of what we now call genes. Which of the following did Mendel assume to be true?
a. Genes are carried on chromosomes
b. Genes segregate independently in crosses
c. Recombination occurs at random positions between genes
d. Genes are made of deoxyribonucleic acid
e. Genes encode protein products

4. In class we talked about the concept of epistasis. Which of the following is an example of epistasis?
a. A gene controlling flower color can result in either red or white flowers, depending on its DNA sequence
b. The B gene controls coat color in labradors, yet combining homozygous recessive forms of another gene with any combination of B alleles results in a yellow dog
c. The physical distance between two genes on a chromosome is directly related to the frequency of recombination between them
d. A pea plant with white flowers when allowed to self-pollinate produces only plants with white flowers
e. Crossing a plant with red flowers by a plant with white flowers produces plants with pink flowers

5. A male member of the class discovers that some of his mother's maternal uncles suffered from pattern baldness, as do some of her brothers. He has an older brother who also suffers from pattern baldness. None of the women in the family has this problem. He is worried that he will soon have to contact "Hair Club for Men", so you investigate and find that there is no record of pattern baldness in his father's family. You quickly inform him that:
a. He has nothing to worry about, he couldn't have inherited pattern baldness
b. He has one chance in four of inheriting pattern baldness
c. He has one chance in two of inheriting pattern baldness
d. He will certainly inherit pattern baldness
e. You don't have enough information to tell him if he might inherit pattern baldness

6. Early experiments testing the identity of the genetic material were done by Griffith and by the trio of Avery, MacLeod and McCarty. The latter group improved on the work of Griffith in one important way which allowed them to draw an important conclusion about the genetic material. What was that improvement?
a. They used two forms of the bacterium Streptococcus pneumoniae, the smooth and rough types
b. They heat killed the bacterium
c. They used purified DNA in their experiments
d. They tested the ability of cells to kill mice when injected
e. They found that smooth and rough types of the bacterium rarely interconverted spontaneously

7. The result of experiments performed in the late 1950s indicated that the replication of DNA was "semi-conservative". What does this mean?
a. Only part of each DNA strand is replicated
b. The two double-stranded molecules generated by replication both contain one new and one old strand
c. One of the two double-stranded molecules generated by replication is entirely new
d. One strand of each DNA molecule is destroyed during replication, and one is retained
e. After some replication events the original DNA molecule remains unchanged

8. You identify a short region of RNA within a longer mRNA with the following sequence:
5'-GAUUACGCAUCC-3'
What is the sequence of the DNA region from which this is copied (that is, the strand which acts as template for the synthesis of this RNA)?
a. 5'-GGATGCGTAATC-3'
b. 5'-TCGGCATACGAA-3'
c. 5'-CTAATGCGTAGG-3'
d. 5'-GGTAGCGATTAC-3'
e. 5'-GATTACGCATCC-3'

9. The "genetic code" is the list of code words ("codons") in RNA which specify particular amino acids in proteins. Which of the following statements about the genetic code is correct?
a. The genetic code is degenerate
b. Codons are groups of three nucleotides
c. Groups of codons specifying the same amino acid differ in the third position
d. Three of the codons specify peptide termination
e. All of the above

10. Watson and Crick proposed a structure for deoxyribonucleic acid in 1953. Of the following statements which is not a correct description of that structure?
a. The structure involves four nucleotides, symbolized as A, G, C and T
b. Nucleotides are linked together by phosphodiester bonds between the 5' and 3' positions of deoxyribose
c. The two strands of DNA are parallel, that is they run in the same chemical direction
d. The phosphates in nucleotides are on the outside of the structure
e. The overall structure of the molecule is a double helix

11. Genes encode proteins, but those protein products are not necessarily expressed in all circumstances or by all cells. The lac operon provides a simple system to study mechanisms of gene regulation. Which of these statements about the lac system is correct?
a. The genes of the lac system are organized as an "operon"
b. A repressor protein binding to the lac mRNA can stop it from being translated
c. Binding of the CAP·cAMP complex to the lac DNA can stimulate transcription of the lac genes
d. a and b
e. a and c

12. Genes can be regulated in various ways. In class I discussed several mechanisms of gene regulation which are also mentioned in Starr & Taggart. Which one of these statements about genetic control is incorrect?
a. Transcription of some genes is regulated
b. Genes can be regulated by processing of the mRNA after transcription
c. In some cases the ability to translate the mRNA is regulated
d. Some genes are regulated by the specific degradation of their protein product
e. In no case is a gene regulated by more than one of these mechanisms

13. Restriction enzymes are one of the critical elements of the technology we call "genetic engineering". These enzymes are important because:
a. They restrict the growth of potentially harmful bacteria used in "gene cloning" experiments
b. They allow scientists to break DNA down into fragments
c. They modify DNA so that it can not be broken when it enters a foreign host
d. They show no substrate specificity whatsoever
e. They join two DNA molecules together by forming new phosphodiester bonds

14. A new technique called Polymerase Chain Reaction (PCR) has revolutionized molecular biology. The technique can be used to generate fragments of DNA beginning and ending at any position within a larger fragment. What feature of PCR provides this ability?
a. DNA polymerase repeatedly copies DNA strands
b. Two short DNA primers define where replication begins
c. The amount of product formed increases geometrically
d. The DNA products are digested with restriction enzymes at the end of PCR
e. DNA polymerase initiates replication at a promoter

15. In "The Origin of the Species" Darwin proposes a mechanism to explain the origin of the biological diversity on Earth. Which of these statements about Darwin's theory is correct?
a. Darwin believed that his theory of evolution invalidated all religious descriptions of the origin of biological diversity
b. Darwin argued that evolution occurred as a result of inheritance of acquired characteristics
c. Darwin invented the concept of evolution
d. Darwin argued that competition among individuals for limiting resources would select for changes in the frequencies of certain genetic traits
e. Changes in DNA sequence are heritable

16. Catastrophism is a theory which was put forth to explain the fossil record which shows that over time species have been eliminated and replaced by other species not previously present in the record. How does Catastrophism explain this fact?
a. Previously existing unabundant species repopulate earth after most plants and animals are killed by a catastrophe
b. Those plants and animals which survive have special adaptive traits
c. New species arise because competition between individuals selects for genetic changes
d. The catastrophe selects for survival of the animals and plants which are fittest
e. Evolution occurs gradually during periods between catastrophes

17. Modern evolutionary theory differs from the theory created by Darwin in many ways. What idea proposed by modern evolutionary theory was not a part of Darwin's theory?
a. Evolution occurs because of intense competition among individuals
b. Evolution occurs because selection favors the survival of the fittest
c. The processes which drive evolution are often random, completely unrelated to fitness
d. Evolution has occurred in all parts of the world
e. Evolution does not involve inheritance of acquired characteristics

18. The Hardy-Weinberg Rule describes the behavior of genetic traits in a population of individuals in which there are two forms of an allele present. Why is the Hardy-Weinberg Rule useful to population biologists?
a. They can use it to predict how the frequencies of alleles will change as environmental conditions change
b. They can use it to determine how selective pressure on a population is forcing a change in allele frequencies
c. It is not useful to population biologists
d. They can use it to determine the frequency of epistasis in a population
e. They can use it to calculate the frequency of each allele in a population

19. Microevolution can occur as a result of various mechanisms which cause changes in allele frequency in a population. Which of the following is an example of one of those mechanisms called "genetic drift"
a. Selection operating on a population may tend to favor intermediate phenotypes
b. After a population experiences an event involving massive death the allele frequencies in the population may have changed drastically
c. Individuals moving between two populations tend to reduce the differences in allele frequencies between the two populations
d. Selection operating on a population may tend to favor either of two extreme phenotypes
e. Two allelic traits may be stabilized in a population because the heterozygote have some selective advantage

20. The story of the peppered moth and the effect of environmental pollution on the frequency of allelic types in the English midlands is a classic of population biology. Which of these statements about the peppered moth story is not correct?
a. Light-colored moths decreased in number because of the increased contrast with darkened tree bark
b. The effect of environmental changes on survival of the moths were tested by controlled experiments
c. The change in frequency of the two types of moths occurred because of competition between the moths for a limiting resource
d. When pollution was reduced the frequency of lightation again became adaptive
e. Changes in the frequency of the two types of moths probably depended on their being preyed on by birds

21. The concept of "species" has changed over time. What is the essential aspect of the "biological species concept"?
a. Species all share similar behaviors and appearance
b. All individuals of a species are descended from a common ancestor
c. Individuals in a species all occupy the same role in the ecology of their region
d. Individuals of a species normally mate and produce fertile offspring in the wild
e. All individuals in a species share a common body plan or structure

22. The "Central Dogma" of molecular biology states:
a. All aspects of living things can be explained by interactions among molecules
b. DNA codes for RNA which in turn codes for proteins
c. Deoxyribonucleic acid is the genetic material
d. The expression of genes must be regulated so that they are expressed only in appropriate cells or under appropriate circumstances
e. Dexyribonucleic acid is chemically stable

23. In the Hershey-Chase experiment bacteriophage were labeled with radioactive forms of sulfur (35S) or phosphorus (32P). These atoms were used to follow the bacteriophage protein and DNA as the phage infected a culture of bacteria. The conclusion of the experiment was that:
a. Radioactive elements intefere with virus propagation
b. Proteins enter the bacteriophage by a different route than does DNA
c. After removing the DNA from a bacteriophage it can not infect a bacterium
d. DNA is copied into proteins which are injected into the bacterium
e. DNA must be the genetic material since it enters the bacterium during infection

24. The ribosome plays a fundamental role in the process of translation. What is that role?
a. It is responsible for making an RNA copy of the DNA genome
b. It is the site of decoding of messenger RNAs into proteins
c. It regulates the rate of initiation of RNA synthesis on various genes
d. It is the site at which copies of DNA are synthesized
e. It binds to genes to repress synthesis of messenger RNAs

25. Beginning around the year 1800 scientists became increasingly convinced that species have changed over time. Which of the following was not among the evidence supporting this conclusion?
a. Unexpected similarity in structure which had no obvious purpose (for example, similarity between hands, bat wings, and whale flippers)
b. The existence of increasingly complex fossils over time in the geologic record
c. The existence of unique species in isolated locations, (for example, on islands)
d. The inability to find intermediate types between species over time ("missing links")
e. None of the above


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