Selected Recent Publications

P. Moitra, M. Alafeef, K. Dighe, P. Ray ... D.D. Frey, D. Pan, G. Rao "Rapid and Low-Cost Sampling for Detection of Airborne SARS-CoV-2 in Dehumidifier Condenste" Biotechnol. Bioeng., 118, 3029-3036-123, 2021  [abstract]

P. Rezaei, L.D. Pfefferle, D.D. Frey, "Metal-Semiconductor Sorting of Large-Diameter Single-Wall Carbon Nanotubes by pH-Dependent Binding to a Hydrophobic Interaction Adsorbent" Carbon, 175, 112-123, 2021  [abstract]

R. Adiga, A.U. Andar, S. Borhani, D. Bergenson, S. Deldari, D.D. Frey, X. Ge, Y. Liu, G. Rao, ..., L. Wong, "Manufacturing Biological Medicines on Demand: Safety and Efficacy of GCSF in a Mouse Model of Total Body Irradiation" Biotechnol. Prog., 36(3), e2970, 2020  [abstract]

A.U. Andar, S. Deldari, E. Gutierrez, D. Bergenson, M. Al-Adhami, C. Gurramkonda, L . Telosa, Y. Kostov, D. D. Frey, G. Rao, "Low Cost Customizable Microscale Toolkit for Rapid Screening and Purification of Therapeutic Proteins" Biotechnol. Bioengin., 116, 870-881, 2019  [abstract]

Y. Liu, S. Deldari, H. Guo, C.R. Narahari, R.C. Bates, R. Swanson, S. Ghose, Z.J. Li, D.D. Frey "Evaluation of Chromatofocusing as a Capture Method for Monoclonal Antibody Products" J. Chromatogr. A, 1568, 108-122, 2018.  [abstract]

B.C. Bundy, J.P. Hunt, M.C. Jewett, J.R. Swartz, D.W. Wood, D.D. Frey, G. Rao "Cell-Free Biomanufacturing" Current Opinion Chemical Engineering, 22, 177-183, 2018.  [abstract]

R. Adiga, M. Al-Adhami, A. Andar, S. Borhani, S. Brown, D. Bergenson, M. A. Cooper, S. Deldari, D. D. Frey, X. Ge, H. Guo, C. Gurramkonda, P. Jensen, Y. Kostov, W. LaCourse, Y. Liu, A. Moriera, ..., G. Rao, J. Wilhide, D. Wood, A. Zuber "Point-of-Care Production of Therapeutic Proteins of Good-Manufaturing-Practice Quality" Nature Biomedical Engineering, 2, 675-686, 2018.  [abstract]

C. Gurramkonda, A. Rao, S. Borhani, M.A. M. Pilli, S. Deldari, X. Ge, N. Pezeshk, T.-C. Han, M. Tolosa, Y. Kostov, L. Tolosa, D.W. Wood, K. Vattem, D.D. Frey, G. Rao "Improving the Recombinant Human Erythropoietin Glycosylation using Microsome Supplementation in CHO Cell-Free System" Biotechnol. Bioeng., 115, 1253-1264, 2018.  [abstract]

K. Tran, C. Gurramkonda, M.A. Cooper, M. Pilli, J.E. Taris, N. Selock, T.-C. Han, M. Tolosa, A. Zuber, C. Penalber-Johnson, C. Dinkins, N. Pezeshk, Y. Kostov, D.D. Frey, L. Tolosa, D.W. Wood, G. Rao "Cell-Free Expression of a Therapeutic Protein: Expression, Purification, and Characterization of Recombinant Streptokinase using a CHO Lysate" Biotechnol. Bioeng., 118, 92-102, 2018.  [abstract]

S. Johnson, K. A. Brorson, D. D. Frey, A K. Dhar, D. A. Cetlin "Characterization of Non-Infectious Virus-Like Particles for Viral Clearance Applications," Applied Biochem. Biotechnol., 183, 318-331, 2017.  [abstract]

N. D. S. Pinto, S. D. Uplekar, A. R. Moreira, G. Rao, D. D. Frey, "Immunoglobulin G Elution in Protein A Chromatography Employing the Method of Chromatofocusing for Reducing the Co-Elution of Impurities," Biotechnol. Bioeng., 114, 154-162, 2017.  [abstract]

C. Penalber-Johnson, X. Ge, K. Tran, N. Selock, N. Sardesai, C. Gurramkonda, M. Pelli, M. Tolosa, L. Tolosa, Y. Kostov, D.D. Frey, G. Rao, ""Optimizing Cell-Free Protein Expression in CHO: Assessing Small Molecule Mass Transfer Effects in Various Reactor Configurations,"" Biotechnol. Bioeng., 114, 1478-1486, 2017.  [abstract]

G. Rao, Y. Kostov, L. Tolosa, L. Hung, X. Ge, D. D. Frey, V. Singh, "Microscale Bioprocessing System and Method for Protein Manufacuring," US Patent 9,388,3732, 2016.  [abstract]

M. M. H. El-Sayed, D. D. Frey, "Chromatofocusing" in Encyclopedia of Life Sciences, Wiley, 2015 [abstract]

N. D. S. Pinto, D. D. Frey, "Displacement Chromatography of Proteins Using a Retained pH Front in a Hydrophobic Charge Induction Chromatography Column," J. Chromatogr. A, 1387, 53-59, 2015.  [abstract]

H. Guo, X. Li, D. D. Frey, "Development of Chromatofocusing Techniques Using Mixed-Mode Column Packings for Protein Separations," J. Chromatogr. A, 1323, 57-65, 2014.  [abstract]

X. Kang, J. P. Kutzko, M. L. Hayes, D. D. Frey, "Monoclonal Antibody Heterogeneity Analysis and Deamidation Monitoring with High-Performance Cation-Exchange Chromatofocusing Using Simple, Two Component Buffer Systems," J. Chromatogr. A, 1283, 89-97, 2013.  [abstract]

D. D. Frey, H. Guo, N. Karnik, "High Performance Liquid Chromatography in the Undergraduate Chemical Engineering Laboratory," Chem. Eng. Educ. 47, 15-24, 2013.  [abstract]

H. Guo, D. D. Frey, "Interpreting the Difference Between Conventional and Bi-directional Plate Height Measurements in Liquid Chromatography," J. Chromatogr. A, 1217, 6214-6229, 2010.  [abstract]

G. Rao, Y. Kostov, A. Moriera, D. Frey, M. Hanson, O.-W. Rief, R. Baumfalk, J. Qualitz, "Non-Invasive Sensors as Enablers of Smart Disposibles," BioProcess International, 7, Supl. 1, 24-27, 2010. [abstract]

D. M. Strauss, S. Lute, Z. Tebaykina, D. D. Frey, C. Ho, S. G. Blank, K. Brorson, Q. Chen, B. Yang, "Understanding the Mechanism of Virus Removal by Q Sepharose Fast Flow Chromatography During the Purification of CHO-Cell Derived Biotherapeutics, " Biotechnol. Bioeng. 104, 371-380, 2009.  [abstract]

H. Shen, D. D. Frey, "Serial Displacement Chromatofocusing and its Applications in Multidimensional Chromatography and Gel Electrophoresis: Part I. Theory and General Considerations," J. Chromatogr. A, 1216, 967-976, 2009.  [abstract]

H. Shen, X. Li, C. J. Bieberich, D. D. Frey, "Serial Displacement Chromatofocusing and its Applications in Multidimensional Chromatography and Gel Electrophoresis: Part II. Experimental Results," J. Chromatogr. A, 1216, 977-986, 2009.  [abstract]

K. Brorson, H. Shen, S. Lute, J. Soto Pérez, D. D. Frey, "Characterization and Purification of Bacteriophages Using Chromatofocusing," J. Chromatogr. A, 1207, 110-121, 2008. [abstract]

H. Shen, X. Li, C. J. Bieberich, D. D. Frey, "Reducing Sample Complexity in Proteomics by Chromatofocusing with Simple Buffer Mixtures," Methods. Mol. Biol., 424, 187-203, 2007. [abstract]

X. Ge, M. Hanson, H. Shen, Y. Kostov, K.A. Brorson, D. D. Frey, A. R. Moreria, G. Rao, " Validation of an Optical Sensor-Based High-Throughput Bioreactor System for Mammalian Cell Culture," J. Biotechnol., 22, 293-306, 2006. [abstract]

D. D. Frey, X. Kang, "New Concepts in the Chromatography of Peptides and Proteins," Curr. Opin. Biotechnol., 16, 552-560, 2005. [abstract]

J. Soto Pérez, D. D. Frey, "Behavior of the Inadvertant pH Transient Formed by a Salt Gradient in the Ion-Exchange Chromatography of Proteins," Biotechnol. Progr., 21, 902-910, 2005. [abstract]

H. Shen, D. D. Frey, "Effect of Charge Regulation on Steric Mass-Action Equilibrium for the Ion-Exchange Adsorption of Proteins," J. Chromatogr. A, 1079, 92-104, 2005. [abstract]