OBJECTIVE

 

To find a challenging research and development position which would profit from my technical skills as a biochemical engineer.  Specifically desire to  apply engineering principles to bioseparation processes, utilize and increase knowledge of cGMPs, and facilitate bioprocess development.

EDUCATION

	Ph.D. in Chemical Engineering, Expected: 2002, GPA 3.6/4.0 University of Maryland, Baltimore County (UMBC), Baltimore, Maryland
	M.S. in Biochemical Engineering, 07/98, GPA 86/100  Chinese Academy of Sciences (CAS), Beijing, China
	B.S. in Microbiology, 07/95, GPA 90/100  Shandong University, Jinan, China
	Biotechnology Regulatory Program Certificate, UMBC Regulatory Issues in Biotechnology (Fall, 1999)  Good Manufacturing Practices for Bioprocesses (Spring, 2000)  Quality Control/Quality Assurance of Biotechnology Products (Fall, 2000) Biotechnology GMP Facility Design, Construction and Validation (Spring, 2001)
	Computer Science Minor Certificate, 07/95, Shandong University

TECHNICAL SKILLS

	Separation and Purification Using HPLC and FPLC: SEC, IEC, RPC, HCIC, chromatofocusing, affinity chromatography, and 2D-HPLC.
	Cell Culture and Fermentation: Plant cell culture, bacterial and fungal fermentation using standard stirred tank reactor (STR), air-lift bioreactors and other novel bioreactors.
	Analytical Techniques: MS, IEF, SDS PAGE, native PAGE, 2D-PAGE, Western blot, and ELISA.
	Computer Skills: Website design, Database, Microsoft Word, Excel, PowerPoint, and Access.
	Other Skills: Proteomics, packing HPLC and FPLC columns, bioreactor design, and bioprocess scale-up.

 

PROFESSIONAL EXPERIENCE

	Analytical- and Preparative-Scale Protein Purification using Novel Chromatofocusing Methods  PhD thesis, 08/98 to present Fast (2 minutes or less) high-resolution separation of proteins at both analytical- and preparative-scales using simple buffer mixtures.  Developed computer software to design a pH gradient of a desired shape.  Developed novel methods such as cation-exchange chromatofocusing and hydrophobic charge induction chromatofocusing to extend the range of application of chromatofocusing.
	Characterization of Novel Chromatographic Column Packings Collaborative project with Grace Vydac, 08/00 to present Investigated a novel silica-based column packing to be used in affinity chromatography of monoclonal antibodies.  Characterized novel types of column packings, optimized the particle size, pore size, protein A loading, and prepared the technical parameters used for marketing.
	Production of Scleroglucan by Sclerotium glucanicum in an Air-Lift Bioreactor with an Internal Loop   Master’s thesis, 07/96 to 07/98 Designed and successfully used a novel air-lift bioreactor with an internal loop (ALR-IL) in the production of scleroglucan, a type of polysaccharide with high anti-tumor activity. Studied effect of air flow rate on the synthesis of scleroglucan. Investigated the mixing behavior and hydrodynamic characteristics of the highly viscous fungal broth in ALR-IL. Based on these understandings, the operation conditions were optimized to reach a high level of polysaccharide productivity compared to that in STR.
	Artemisinin Production by Hairy Roots and Adventitious Shoots of Artemisia annua L. in a Novel Mist Bioreactor   Additional work done at CAS, 09/95 to 07/96 Cultured hairy roots and adventitious shoots of Artemisia annua L. in a novel mist bioreactor specifically designed for plant cell culture. Investigated effect of light, temperature and cultivation modes on growth and artemisinin synthesis.
	Optimization of 2-Keto-L-Gulonic Acid (2KGA) Fermentation Process in the Mixture Culture of  Vitamin C Two-Step Fermentation    Bachelor’s thesis done at Tsinghua University, Beijing, China, 02/91 to 07/91  Clarified the previously unknown relationship between the two kinds of bacteria, Bacillus megaterium and Gluconobacter oxydans, in the mixture culture system for 2KGA fermentation. Tested the influence of B. megaterium on the population growth of G. oxydans and 2KGA production at different ratios of initial inoculation densities.

PUBLICATIONS
 

	1.  X. Kang and D. D. Frey “High Performance Strong-Base Ion-Exchange Chromatofocusing of Proteins and Peptides” (in preparation).
	2.  X. Kang, Y. Wang and B. McNeil “Kinetic Models of Scleroglucan Fermentation in a Standard Stirred Tank and Air-Lift Bioreactors” (in preparation).
	3.  X. Kang and D. D. Frey “Cation-Exchange Chromatofocusing” J. Chromatogr. A (Submitted).
	4.  X. Kang and D. D. Frey “Chromatofocusing Using Micropellicular Column Packings with Computer-Aided Design of the Elution Buffer Composition,” Analytical Chemistry, 74, 1038-1045, 2002.
	5.  X. Kang, H. Wang, Y. Wang, L. M. Harvey, and B, McNeil “Hydrodynamic Characteristics and Mixing Behaviour of Sclerotium glucanicum Culture Fluids in an Airlift Reactor with an Internal Loop Used for Scleroglucan Production,” J. Industrial Microbiology and Biotechnology, 27(4), 208-214, 2001.
	6.  R. Bates, X. Kang, and D. D. Frey “High Performance Chromatofocusing Using Linear and Concave pH Gradients Formed with Simple Buffer Mixtures  I. Effect of Buffer Composition on the Gradient Shape,”  J. Chromatogr. A, 890, 25-36, 2000.
	7.  X. Kang, R. Bates, and D. D. Frey "High Performance Chromatofocusing Using Linear and Concave pH Gradients Formed with Simple Buffer Mixtures  II. Separation of Proteins," J. Chromatogr. A, 890, 37-43, 2000.
	8.  X. Kang, Y. Wang, L. M. Harvey and B. McNeil "Effect of Air Flow Rate on Scleroglucan Synthesis by Sclerotium glucanicum in an Airlift Bioreactor with an Internal Loop," Bioprocess Engineering, 23(1), 69-74, 2000.
	9.  C. Liu, Y. Wang, X. Kang, F, Ouyang, C. Ye, and F. Li "Investigation of Light, Temperature and Cultivated Modes on Growth and Artemisinin Synthesis of Artemisia annua L. Shoots,"Acta-Phytophysiologica-Sinica, 25(2), 105-109, 1999.
	10.  C. Liu, Y. Wang, X. Kang, F, Ouyang, C. Ye, and F. Li "Artemisinin Production by Adventitious Shoots of Artemisia annua in a Novel Mist Bioreactor," Acta-Botanica-Sinica, 41(5), 524-527, 1999.


 
 

 



 

---


Last Modify: 08/01/02