B.S. (1983) Bucknell University
The research we do in our lab is in the area of cellular engineering. We use a variety of engineering tools to analyze reactions that occur on the surface of cells and/or within cells to try to understand the mechanism of various diseases. We then use that information to try to alter the selectivity of those reactions such that we prevent or slow the progression of disease. For example, some proteins, such as beta amyloid of Alzheimer's disease, interact with neurons. If the protein is in a normal or "healthy" conformation, the interaction is benign, while if the protein is in its amyloid or "pathogenic" conformation, the protein-cell interaction leads to cell death. We would like to understand the mechanism of the protein-cell interaction and be able to prevent it.
The following students have worked with me (either advised or co-advised) towards their graduate degrees:
PhD students: Michael Edwards, Roberto Linares, Dawn Rymer, Dina Triyoso, Steven Sheng-Shih Wang, In Hong Yang, and Jennifer Ruiz Pacheco, Sungmun Lee, Wanida Wattanakaroon, James Henry, and Dhara Patel.
Master’s students: Meredith Chapeaux, Rene’ Elms, Vasaliki Kazantzi, Camilo Velez Vega, Suk Joon Yoo, Qi Min Liu and Vinita Mehrotra.
Currently, my former students are working for Proctor and Gamble, Motorola, Exxon-Mobile, OSI Software, Alpha Vax Pharmaceuticals, Pfizer, Taiwan University, Louisiana State University or are students and postdocs in various laboratories at Texas A&M University, Georgia Tech and Johns Hopkins University.
The current research group members include Chris Cowan, Irina Ramos, Ben Keshet, Tony Geisz, James Pallikal, Shannadora Hollis, Izath Aguilar, Deborah Lipman and Debbie Lin.
I am always looking for independent, resourceful and creative people to join the lab to work on all aspects of challenging and important problems associated with human disease. But there should also be time for enjoying all aspects of life in graduate school.
Sailing on the Chesapeake with me: Drs. Jennifer Ruiz and Guillermo Coward Kelly, Irina Ramos, Dr. Dhara Patel, and Professor Castellanos. There are many more sailing pictures.
After a day fishing on the Chesapeake. From right to left, Tony Geisz, Irina Ramos, Laura Ciedera, James Pallikal, Ben Keshet, and me. We all caught rockfish, but only some of us chose to "show off" our catch.
The department takes an annual ski trip. John Kim and his wife, Professor Frey, Chris Cowan, Professor Castellanos, Irina Ramos, Silivya Petrovka, Michael Johnson, Professor Marten and son (Max) and me.
Chris Cowan and Tony Geisz, in the foreground of the picture, have won the department bocce tournament two years in a row.
have several ongoing research projects....
Engineering of Drug Delivery to T Cells
T cells are one of the cellular components of the immune system. Inappropriate stimulation of a T cell by a normal protein results in healthy tissue destruction. This mechanism is thought to be responsible for a number of diseases, including some forms of arthritis and diabetes. We are constructing mathematical models of T cell response and using them to help in the design of antisense and photodynamic therapies to treat T cell diseases.
Exploration of the Role of Biomechanical Forces in Neurodegeneration
A number of diseases affecting central nervous system tissue, such as glaucoma, head injury, and hydrocephalus, are characterized by elevation of intraocular or intracranial pressure and/or local shear stress and strain. We propose that the neurodegeneration in these diseases is a direct result of the local shear stress and strain acting on neurons. My students are developing an in vitro model of shear stress and strain induced neurodegeneration which can be used to elucidate steps in the mechanism of shear stress and strain induced neurodegeneration and to test new compounds for the treatment of diseases such as glaucoma and head injury.
Protein Aggregation and Amyloid Diseases
At least 16 different human proteins can fold abnormally resulting in the formation of an amyloid fibril and some accompanying pathology. Two of the best known examples of these diseases are Alzheimer's disease and transmissible spongiform encephalopathies (such as Mad Cow disease). We are investigating the mechanism by which amyloid-forming proteins cause neurodegeneration and exploring the relationship between peptide structure and biolgoical activity. We are also developing biomimitic polymers as potential therapeutics for use in the prevention of amyloid toxicity. Our research could lead to the development of cures for these otherwise fatal diseases. In addition, we, along with Dr. Gerard Cote’ from Texas A&M University, are trying to exploit our knowledge of these disease causing proteins to develop novel sensors for various amyloid diseases.
Cells Under Stress
D. Triyoso and T. Good. Pulsatile Shear Stress leads to DNA Fragmentation in Human SH-SY5Y Neuroblastoma Cell Line. J. Physiology , 515: 355-365, 1999.
M. Edwards, S-S Wang, and T. Good. Role of Viscoelastic Properties of Differentiated SH-SY5Y Human Neuroblastoma Cells in Cyclic Shear Stress Injury. Biotechnology Progress 17: 760-767, 2001.
M. Edwards and T. Good. Use of a Mathematical Model to Estimate Stress and Strain During Elevated Pressure Induced Lamina Cribrosa Deformation. Current Eye Research. 23: 215-225, 2001.
S.-S. Wang and T. Good. Effect of Culture in a Rotating Wall Bioreactor on the Physiology of Differentiated Neuron-like PC12 and SH-SY5Y Cells, J. Cellular Biochemistry. 83: 574-584. 2001.
D. Rymer and T. Good. The Role of Prion Peptide Structure and Aggregation in Toxicity and Membrane Binding. J. Neurochem, 75: 2536-2545, 2000.
D. Rymer and T. Good. Amyloidogenic b(1-40), b (25-35) and Bovine Calcitonin Are Toxic Via A Common Pathway Involving G Protein Activation. J. Biol. Chem., 276: 2523-2530, 2001.
S.-S. Wang, D. Rymer and T. Good. Cholesterol and Sialic Acid Removal Protects Cells from the Toxic Effects of b-Amyloid Peptides. J. Biol. Chem. 276: 42027-42034, 2001.
S.-S. Wang, A. Becerra-Arteaga, and T.Good. Development of a novel diffusion-based method to estimate the size of the aggregated Ab species responsible for neurotoxicity. Biotechnology and Bioengineering, 80, 50-59, 2002.
S.-S. Wang, V. Kazantzi and T. Good. Kinetic analysis of the mechanism of b-amyloid induced G protein activation. J Theoretical Biology. 221: 269-278, 2003.
S.-S. Wang, S. Tobler, T. Good, and E. Fernandez. Hydrogen exchange-mass spectrometry analysis of b-amyloid peptide structure. Biochemistry. 42: 9507-14, 2003.
S. Lee, K. Carson, A. Ficht., and T. Good. Hsp20, a novel a-crystallin, prevents Ab fibril formation and toxicity. Protein Science. 14: 593-601,2005.
S. Lee, K. Carson, A. Ficht and T. Good. Small heat shock proteins differentially affect Ab aggregation and toxicity. BBRC 347: 527-533, 2006.
D. Patel, J. Henry and T. Good. Attenuation of beta-amyloid
induced toxicity by sialic acid-conjugated dendritic polymers. BBA General Subjects
1760: 1802-1809, 2006
D. Patel and T. Good. A rapid method to measure b-amyloid induced toxicity in vitro. J. Neuroscience Methods 161: 1-10, 2007.
S. Lee, E. Fernandez and T. Good.
Role of aggregation conditions in structure, stability and toxicity of
intermediates in the Ab
fibril formation pathway.
Protein Science. 16: 723-732, 2007.
D. Patel, J. Henry and T. Good. Attenuation of beta-amyloid induced toxicity by sialic acid-conjugated dendrimers: role of sialic acid attachment. Brain Research (accepted), 2007.
R. Linares, J. Ruiz Pacheco, and T. Good. Efficacy of Different Targeting Agents in the Photolysis of Interleukin-2 Receptor Bearing Cells. J Photochemistry and Photobiology, B. 77: 17-26, 2004.
B. Jung, S. Lee, I.H. Yang, T. Good, and G. Cote’. Automated On Line Noninvasive Optical Glucose Monitoring in Cell Culture System, Applied Spectroscopy. 56: 511-57, 2002.
J. Henry, A. Anand, M. Chowdhury, G. Cote’, R. Moreira, and T. Good. Development of a nanoparticle based - surface modified fluorescence assay for the detection of prion proteins. Analytical Biochemistry. 334:1-8, 2004.
A. Simonian, T. Good, S.-S. Wang, and J. R. Wild. Nanoparticles-based Optical Biosensor for Direct Detection of Organophosphate CW Agents and Pesticides. Analytica Chimica Acta. 534: 69-77, 2005.
A. Anand, R. Moreira, J. Henry, M. Chowdhury, G. Cote' and T. Good. A bio-sensing strategy for the detection of prions. LWT - Food Science and Technology. 38: 849-858, 2005.
H. Beier, C. Cowan, I.H. Chow, J. Pallikal, J. Henry, M. Benford, J. Jackson, T. Good, and G. Cote’. Application of Surface Enhanced Raman Spectroscopy for Detection of Beta Amyloid Using Nanoshells. Plasmonics. 2: 55-64, 2007.
N. Kazantzis and T. Good. Invariant Manifolds and the Calculation of the Long Term Asymptotic Response of Nonlinear Processes Using Singular PDEs. Comput. Chem. Eng. 26: 999-1012, 2002.
M. Rodriguez, T. Good, M. Wales, J. Hua, and J. Wild. Modeling Allosteric Regulation of de novo Pyrimidine Biosynthesis in Escherichia coli. J. Theoretical Biology . 234: 299-310, 2005.
N. Kazantzis, N. Huynh, and T. Good.
A model based characterization of the long term asymptotic behavior of
non-linear discrete time processes using invariance functional equations.
Computers in Chemical Engineering. 29:2346-2354, 2005.