This is a NOESY of S. mitis J22 polysaccharide, both NOE and long range 3JCH show that it exits in multiple conformers. By the way, the background music was played on Bruker NMR by Walter Bauer



Reference:
1. Q. W. Xu, S. Mohan & C. A. Bush "A flexible model for the cell wall polysaccharide of S. sanguis J22 determined by three dimensional 13C filtered NOESY spectra and 13C-1H long range coupling constants combined with molecular modeling" Biopolymers 38, 339-353 (1996)
2. Q. W. Xu, R. Gitti, and C. A. Bush "Comparision of NMR and Modeling Results for a Rigid and a Felxible Oligosaccharide" Glycobiology 6, 281-288 (1996)