To pursue a challenging career as a scientist in the biotechnology or pharmaceutical industry

• Drug and formulation development
• Process development and scale-up
• Receptor-ligand interactions

1994-1999, Chemical and Biochemical Engineering, University of Maryland Baltimore County, Baltimore, MD (Expected graduation date: August, 1999)

1989-1993, Chemical Engineering, University of Roorkee, Roorkee, India.

1996-Present, University of Maryland Baltimore County, Baltimore, MD.

• Established the adhesion kinetics of a pathogenic strain of Staphylococcus aureus to collagen under fluid shear conditions
• Demonstrated for the first time, the profound effect fluid shear has on the efficacy of potential vaccines (adhesion blockers) for septic arthritis and osteomyelitis
• Developed a new technique to quantify cell surface receptor proteins and established the temporal pattern of expression of receptors known to be critical in pathogenesis of infections
• Characterized a novel bacterial adhesion system, thereby demonstrating the feasibility of using bacteria to study receptor-mediated dynamic adhesion. This is a superior alternative to mammalian cells that are currently employed to study the phenomenon

1994-1996, University of Maryland Baltimore County, MD.

• Supervised and graded senior level undergraduate unit operations laboratory class and senior level process control class

1993-1994, Petrochemicals Division, Reliance Industries Ltd., Bombay, India.

Measurement of dynamic cell adhesion in a parallel plate flow chamber using Video Microscopy (Phase contrast and Fluorescence) and Digital Image Processing

Immuno-fluorescence and Flow Cytometry, Chromatography (HPLC, FPLC), SDS and Non-denaturing PAGE, ELISA

Bacterial Cell Culture, Mammalian cell culture, Affinity Chromatography, Papain Digestion, Lyophilization

Conjugation chemistries to immobilize proteins to different solid supports and couple proteins and peptides with fluorescent labels

Programming and Graphical Languages: Fortran, Macro, HTML, MATLAB Operating Systems: MS-DOS, Mac OS and MS Windows

Four Course Certification Program for Biochemical Regulatory Engineering Track (Regulatory Issues in Biotechnology, GMPs for Bioprocesses, QC/QA for Biotech. Products, Biotech. GMP Facility Design Construction and Validation), Immunology, Tissue Engineering, Protein Engineering, Comprehensive Biochemistry, Introduction to Biomedical Engineering, Biochemical Engineering

N. Mohamed, Teeters, M. A., Patti, J. M., Höök, M., Ross, J. M., ‘Inhibition of Staphylococcus aureus adherence to collagen under dynamic conditions’, Infection and Immunity. 1999. Vol. 67, No.2, 589-594.

N. Mohamed, Rainer, T., Ross, J. M., ‘A novel bacterial adhesion experimental model to study the effect of various critical parameters on the phenomenon of receptor-mediated dynamic adhesion’, Biotechnology and Bioengineering (in review).

N. Mohamed, Speziale, P., Ross, J. M., ‘Quantification of the temporal expression of S. aureus cell surface adhesins using flow cytometry’, In preparation.

N. Mohamed, Ross J. M, ‘A novel experimental system to study receptor mediated dynamic adhesion’, Oral Presentation, Annual Meeting of the Mid-Atlantic Biochemical Engineering Consortium, University of Virginia, Charlotesville, April 1999.

N. Mohamed, Ross J. M, ‘Effect of various parameters on dynamic adhesion of S. aureus to collagen’, Poster, Becton Dickinson Life Sciences Symposium, UMBC, November 1998.

N. Mohamed, Ross, J. M., ‘Recombinant adhesin peptides inhibit adhesion of S. aureus to collagen under dynamic conditions’, Poster, Annual Meeting of the Biomedical Engineering Society, San Diego, California October, 1997.

N. Mohamed, and Ross, J. M., ‘Adhesion of S. aureus to collagen under dynamic conditions’, Poster, Annual Meeting of the American Institute of Chemical Engineers, Chicago, Illinois, November 1996.

Available on request