M.S., Biological Sciences,
email@example.com; FAX (410)
455-3875; Voice (410) 455-2146/3581
Teaching & Research Interests -
Applied Molecular Biology:
I am the instructor for the Advanced Molecular
Biology laboratory courses that are the key components of the nine-month
Master's Degree Program in Applied Molecular Biology. The laboratory courses are designed to teach students
how to work in a molecular biology research laboratory in preparation for
an industrial or academic laboratory position or advanced graduate work.
Students learn basic and advanced molecular techniques in the context of
research projects as well as designing experimental strategies and
controls, and keeping a laboratory notebook. We have weekly lab meetings
to discuss strategies, data, and current journal articles. Over the years,
we have worked on many different research projects including:
- Cloning, expression, and binding site determination of the C.
elegans transcription factor, egl-18.
- Optimization of codon usage for expression of the human NKX3.1 gene in
E. coli using gene assembly PCR.
- Cloning, expression and functional analysis of the human RGR protein
in COS cells.
- Comparative sequence analysis of the catsup gene in sister
- Construction of mutant and epitope-tagged variants of the nitA gene
from Volvox for use in transformation.
- Construction of MHC Class II-expressing and her2 antigen-expressing
retroviral constructs for use in breast cancer vaccine research
- Development of methods for detecting SNP's in genomic samples of bird
species using real-time PCR
- Determination of ddc expression levels in Drosophila
melanogaster in response to wounding
- Cloning and characterization of a neuronal cytoskeleton protein from
- Cloning of carbonic anhydrase from Chlamydomonas reinhardtii
- Construction of vectors for production of IL-1-beta in mouse tumor
- Generation of cell lines expressing a number of different
- The isolation and characterization of rhodopsin and other opsins
from both striped bass
- The expression, purification and functional analysis of
rhodopsin-expressing COS cells
- The generation of polyclonal antisera against crayfish and
My interests also lie in Science Education and, in particular,
biotechnology and recombinant DNA at the high school level. I have
taught workshops in biotechnology as part of high school teacher
professional development for many years, most of these workshops were
funded by the Maryland Higher Education Commission and others were part of
the Maryland Governor's Academy for Math and Science. All of these
workshops are "hands-on" experience in recombinant DNA.
Additionally, I have taught Advanced Recombinant DNA methods for scientist
and other science-related professionals during the summer.
Selected Publications and
Wolf, J. B., 2003. Inefficient translation and replication. Human
Molecular Biology Laboratory Manual: Book Review. Heredity.
Deutch, A. D., David V. A.
and J. B. Wolf. 1994. Enhanced Gene Expression in Response to Lactation
Signals. U. S. Patent # 5,320,952.
Leonard W. J., J. B. Wolf, and. N. F. Halden. Dec 17, 1991. Assay for
Mycoplasma Fermentans. U.S. Patent #5073486.
Leonard W. J., N. F. Halden,
and J. B. Wolf. 1990. Novel Restriction Endonuclease. U. S. Patent #
Halden, N. F., J. B. Wolf
and W. J. Leonard. 1989. Identification of a Novel Site Specific
Endonuclease Produced by Mycoplasma fermentans: Discovered While
Characterizing DNA Binding Proteins in T-lymphocyte Cell Lines. Nucleic
Acids Research, 17:3491-3499.
Cross, S. L., M. B.
Feinberg, J. B. Wolf, N. J. Holbrook, F. Wong-Staal, W. J. Leonard. 1987.
Regulation of the Human Interleukin-2 Receptor Alpha Chain Promoter:
Activation of a Nonfunctional Promoter by the Transactivator Gene of
HTLV-I. Cell, 49:47-56.
Brey, R. N., C. D. B.
Banner, and J. B. Wolf. 1986. Cloning of Multiple Genes Involved with
Cobalamin (Vitamin B12) Biosynthesis in Bacillus megaterium.
Journal of Bacteriology, 167:623-630.