| GFP applications site which has enoumous information and links about GFP | http://pantheon.cis.yale.edu/~wfm5/gfp_gateway.html |
| GFP news group--not easy to use | http://www.bio.net/hypermail/FLUORESCENT-PROTEINS/ |
| A resource paged for teachers: use GFP to teach molecular biology | http://141.106.116.94/~herman/science_ed/Pages/gfp/index.html |
| Suplier of GFP constructs, some of the user manuals are useful too | http://gfp.clontech.com/ |
| Biochemistry of GFP/BFP: slide show! | http://lyco.lycoming.edu/~newman/research/students/georgy/index.htm |
| A brief introduction of GFP | http://www.physiol.usyd.edu.au/billp/greenfluor.html |
| The Molecular Structure of Green Fluorescent Protein--an on-line paper | http://www.bioc.rice.edu/Bioch/Phillips/Papers/gfpbio.html |
| Extensive applications of GFP on bioprocess monitoring and control | http://www.umbi.umd.edu/~cab/bentley/research.html |
| GFP-tagged gene expression etc. | http://spot.Colorado.edu/~klym/ |
| Structure and function of GFP: also a web-based project! | http://public-1.cryst.bbk.ac.uk/PPS2/projects/jonda/ |
| Another link to GFP links! Not a lot, but very useful | http://loki.stockton.edu/~yorkk/York/York/GFPv2.html |
| Bioluminescene & Biothechnology center in Rutgers Univ.: they deal with GFP and holds conferences! | http://aesop.rutgers.edu/~crebb/ |
| Roger Tsien's page. He uses GFP for FRET. He is a big guy in the field | http://www.biology.org/science/neurosci/tsien.htm |
| This is my lab! We've done a lot with GFP' application--on sensor & on cellular expression | http://www.umbc.edu/engineering/cbe/rao.html |
| Herpes-GFP fusions transfected to Dog Osteosarcoma Cells. Nice pictures | http://www.ahabs.wisc.edu/Staff/Harms-j/Herpes.html |
| Nice work about protein secretion&sorting in T.
gondii . Shows the power of GFP |
http://www.sas.upenn.edu/~striepen/gfp.html |
| This link contains some GFP expression constructs. They work well in Xenopus | http://www.imolbio.oeaw.ac.at/xenopus/Marker_pages/PlasMaps/GFP.html |
| Human embryonic kidney cells transfected with GFP gene. They glow! | http://www.neurophys.wisc.edu/~cozzi/cells.html |
| Introduction of GFP, also include products, links & references. Great to start. | http://www.image1.com/info/GFP/ |
| website for international symposium on GFP | http://www.rci.rutgers.edu/~meton/GFP_Symposium.html |
References:
Chalfie, M., Tu, Y., Euskirchen, G., Ward, W.W., and Prasher, D.C. (1994) Green fluorescent protein as a marker for gene expression. Science 263:802-805. Heim R, Prasher DC, Tsien RY (1995) Improved green fluorescence. Nature 373:663-664. Heim R, Tsien RY (1996) Engineering green fluorescent protein for improved brightness, longer wavelengths and fluorescence resonance energy transfer. Current Biology 6:178-182. Albano CR, Randers-Eichhorn L, Bentley WE, and Rao G 1998. Green fluorescent protein as a real time quantitative reporter of heterologous protein production. Biotechnol. Prog. 14: 351-354. Albano CR, Randers-Eichhorn L, Chang Q, Bentley WE and Rao G 1996. Quantitative measurement of green fluorescent protein expression and chromophore cyclization. Biotechnol. Tech. 10: 953-958. Cha HJ, Pham MQ, Rao G, Bentley WE 1997. Expression of green fluorescent protein in insect larvae and its application for foreign protein production. Biotechnol. Bioeng. 56: 239-247. Cha HJ, Wu CF, Valdes JJ, Rao G, Bentley WE 2000. Observations of green fluorescent protein as a fusion partner in genetically engineered Escherichia coli: Monitoring protein expression and solubility. Biotechnol. Bioeng. 67:565-574 Chalfie M, Kain S 1998. Green fluorescent protein: properties, applications, and protocols. New York: Wiley-Liss. DeLisa MP, Li J, Rao G, Weigand WA and Bentley WE 1999. Monitoring GFP-operon fusion protein expression during high cell density cultivation of Escherichia coli using on-line optical sensor. Biotechnol. Bioeng. 65: 54-64. Poppenborg L, Frieh K, Flaschel E 1997. The green fluorescent protein is a versatile reporter for bioprocess monitoring. J. Biotechnol. 58: 79-88. Prasher DD, Eckenrode VK, Ward WW, Prendergast FG, Cormier MJ 1992. Primary structure of the Aequorea victoria green-fluorescent protein. Gene. 111:229-233. Shimomura O, Johnson FH, Saiga Y 1962. Extraction, purification and properties of Aequorin, a bioluminescent protein from the luminous hydromedusan, Aequorea. J. Cell. Comp. Physiol. 59: 223-239 Randers-Eichhorn L, Albano CR, Sipior J, Bentley WE, and Rao G 1997. On-line green fluorescent protein sensor with LED excitation. Biotechnol. Bioeng. 55:921-926. Casper SJ, Holt CA (1996) Expression of the green fluorescent protein-encoding gene from a
tobacco mosaic virus-based vector. Gene 173 (1 Spec No): 69-73.Plautz JD, Day RN, Dailey GM, Welsh SB, Hall JC, Halpain S, Kay SA (1996) Green fluorescent protein and its derivatives as versatile markers for gene expression in living Drosophila melanogaster, plant and mammalian cells. Gene 173 (1 Spec No): 83-87 Stearns T (1995) The green revolution. Current Biology 5:262-264.