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Introduction
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General
Applications of GFP
Quantitative
monitoring of gene expression
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GFP-based High-throughout drug Screening
Application of GFP in drug screening. This is an example where GFP
is cloned under control of the oxidative stress promoter SoxS. Therefore,
when the cells are exposed to stress, they will turn on the SoxS promoter
and express GFP. Thus more stress will result in more GFP expression.
Experiments were carried out in 96-well plates (duplicate in each plate)
where different concentrations of drugs (Paraquat or DNR in this case)
were added. The results show the quantitative response of the cells to
Paraquat and DNR. Use of GFP brings tremendous time and labor
savings to the screening. |
