back to Project main

Monitoring in E. coli

Monitoring in yeast

Bioprocess control and monitoring

back to Quantitative monitoring of gene expression

Now, with the help of GFP, by fusing it with the protein of interest, or in other cases by expressing GFP itself, on-line and real-time monitoring and control of bioprocess is possible.

• Control and monitoring of gene expression in E coli.
• Monitoring gene expression in yeast
• Monitoring gene expression in insect cells: see reference "Expression of green fluorescent protein in insect larvae and its application for foreign protein production."

 

• Dr. Bentley's lab: Extensive applications of GFP on bioprocess control and monitoring.  A key research lab for quantitative application of GFP
• Dr. Rao's lab:  Another lab exploiting GFP's quantitative applications, with expertise on fluorescence technology and biosenor. Website under construction.

• Albano CR, Randers-Eichhorn L, Bentley WE, and Rao G 1998. Green fluorescent protein as a real time quantitative reporter of heterologous protein production. Biotechnol. Prog. 14: 351-354. 
• Albano CR, Randers-Eichhorn L, Chang Q, Bentley WE and Rao G 1996. Quantitative measurement of green fluorescent protein expression and chromophore cyclization. Biotechnol. Tech. 10: 953-958. 
• Cha HJ, Pham MQ, Rao G, Bentley WE 1997. Expression of green fluorescent protein in insect larvae and its application for foreign protein production. Biotechnol. Bioeng. 56: 239-247. 
• DeLisa MP, Li J, Rao G, Weigand WA and Bentley WE 1999. Monitoring GFP-operon fusion protein expression during high cell density cultivation of Escherichia coli  using on-line optical sensor. Biotechnol. Bioeng. 65: 54-64.