Applications of GFP
monitoring of gene expression
General applications of GFP
"The crucial difference between
GFP and [earlier, widely used dyes] is that it works [better] in live cells
or animals." Roger Tsien, a GFP pioneer.
The fact that GFP's fluorescence
requires no other cofactor: the fluorophore forms from the cyclization
of the peptide backbone, makes GFP extremely useful as a biological marker.
This feature makes the molecule a virtually unobtrusive indicator of protein
position in the cells. And researchers have found that use of GFP
as a tag does not alter the normal function or localization of the fusion
Today, GFP has found its broad
use in almost all organisms and all major cellular compartments.
In cellular biology, GFP has been used as reporter gene, cell marker, fusion
tag. GFP has also been used as an active indicator for protease action,
transcription factor dimerization, calcium sensitivity etc. And in
recent years, biochemical engineers have also found GFP's great ability
to quantitatively monitor gene expression in different organisms.
applications from various links
great attributes of GFP: It's these amazing qualities that make GFP
mouse? GFP could be cloned and expressed in almost all organisms. Here
is a mouse with GFP gene.
plakoglobin The fluorescent feature of GFP combined with the
use of confocal microscopy helps researchers to determine protein localization.
Her is an example where GFP helped to determine nuclear localization of