Quantitative
monitoring of gene expression
Application of Green Fluorescent Protein(GFP) on Gene Expression
This website serves as an internet
guide to the applications
of green
fluorescent protein, with a focus on quantitative
control and monitoring
of gene expression using GFP.
Some of the works from this lab are also presented. Each
section includes a brief introduction, presents related works
and list various links and references.
Introduction
of GFP:
Information about the history of GFP, its biochemistry
and molecular biology(structure, fluorophore formation etc.), and various
links and references.
General
application of GFP
Information about GFP's applications as reporter gene,
cell maker, fusion tag and active indicator. Various links with great pictures
taken from confocal microscopy are listed.
Using
GFP for Quantitative monitoring of gene expression
Information about GFP's application as an quantitative
gene reporter. While scientists have been exploiting GFP's application
in biochemistry and molecular biology, biochemical engineers are making
it as a tool for better control and monitoring. Works from this lab
are presented. References included.
- Why quantitative? --bioprocess issues
- The GFP sensors : an example that works well
- GFP fusion proteins for bioprocess control and monitoring :examples on E. coli, yeast and Insect cells
- GFP-based Highthroughout Screening: an example on drug screening