Beginning Molecular Biology Laboratory Manual
Department of Biological Sciences
CHAPTER 1: General
Laboratory Methods
- Safety
Procedures
Preparation of Solutions
Disposal of Buffers and Chemicals
Equipment
Micropipets
Using a pH meter Autoclave operating procedures Operating instructions for spectrophotometer - Working
with DNA
Sterile Technique
CHAPTER 2: Instructions
for Notebook Keeping
CHAPTER 3: Computer
User's Guide
CHAPTER 2: Instructions for Notebook Keeping
CHAPTER 3: Computer User's Guide
CHAPTER 4: Molecular
Biology Methods
- M.1: Preparation of genomic DNA from bacteria
- M.2: PCR amplification of DNA
- M.3: Restriction enzyme digestion of DNA
- M.4: Phenol/chloroform extraction of DNA
- M.5: Ethanol precipitation of DNA
- M.6: Agarose gel electrophoresis
- M.7: Transformation of E. coli by electroporation
- M.8: Wizard PCR preps DNA purification system
- M.9: Alternate method for purifying DNA from agarose gels
- M.10: Transfection of mammalian cells using Lipofectamine (LTI)
- M.11: Southern blotting
- M.12: RT-PCR Protocol
- M.13: Preparation of sequencing gels
- M.14: Isolation of RNA from mammalian cells using RNAZOL (Teltest)
CHAPTER 5: Tissue
Culture Methods
Last updated on 11/23/2005